![]() ![]() (HIV-1), particularly through the blood supply, led to the rapidĭevelopment in 1985 of diagnostic tests for HIV-1 antibodies. If the HIV-2 EIA is positive, an HIV-2 supplemental testĮfforts to prevent transmission of human immunodeficiency Western blot result is negative or indeterminate, an HIV-2 EIA HIV-2 is recommended only if HIV-2 risk factors are present. Western blot confirms the presence of antibodies to HIV, and ![]() (or another licensed HIV-2 supplemental test). Reactive specimen by HIV-1/HIV-2 EIA should be tested by HIV-1 HIV-2 is performed by means of a combination HIV-1/HIV-2 EIA. The following procedures are recommended if testing for both Infection are present, if clinical evidence exists for HIV diseaseĪbsence of a positive test for antibodies to HIV-1, or if HIV-1īlot results exhibit the unusual indeterminate pattern of gag plus However, when HIV testing is indicated, tests for antibodies toĪnd HIV-2 should be obtained if epidemiologic risk factors for Recommend routine testing for HIV-2 in settings other than blood Persons in the United States is extremely low. Guidelines for serologic testing with combination HIV-1/HIV-2Įpidemiologic data indicate that the prevalence of HIV-2 Persons being tested in settings other than blood centers and This articleĬDC recommendations for the diagnosis of HIV-1 and HIV-2 infections Type 2 (HIV-2) beginning no later than June 1, 1992. The Food and Drug Administration (FDA) has recommended thatĭonated blood be screened for antibodies to human immunodeficiency This report was prepared by the following: Please note: This guideline document is obsolete and may not reflect current evidence or best practice and likely contains out-of-date information. Testing for Antibodies to Human Immunodeficiency Virus Type 2 For assistance, please send e-mail to: Type 508 Accommodation and the title of the report in the subject line of e-mail. Contact the University of Washington Clinical Virology Lab (206 685-8037) for ordering instructions.Persons using assistive technology might not be able to fully access information in this file. CSF antibody testing will be performed ONLY when paired with a serum sample. For accurate seroconversion determination, the acute and convalescent samples should be drawn at least 12 -16 weeks apart.įor CSF, see HSV SemiQnt Rapid PCR, Swab/CSF (Herpes Simplex Virus by PCR). It has not been cleared or approved by the US Food and Drug Administration.įor Paired samples, see Herpes Simplex Seroconversion Panel by Western Blot (Paired) for seroconversion on paired serum samples. This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. HSV2 - Positive, Negative or Indeterminate.HSV1 - Positive, Negative or Indeterminate.The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins to which the patient's immune system has antibody. Antibodies, which bind to the viral proteins, are detected by an enzyme-mediated color change. The strips of paper or "blots" containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. HSV1 and HSV2 proteins from detergent lysates ("Bernstein's lysate") of HSV infected cells are separated by electrophoresis and transferred to nitrocellulose paper. The detection of HSV1 and HSV2 IgG class antibodies by Western blot in serum or CSF. ![]()
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